zebrafish-specific microarray platform arrayxs zebrafish v1 zv9  (OakLabs Inc)

Journal: Human Molecular Genetics

Article Title: PITX2 deficiency and associated human disease: insights from the zebrafish model

doi: 10.1093/hmg/ddy074

Figure Lengend Snippet: Microarray target analysis. (A) Heatmap of selected transcripts identified by microarray as potential downstream targets of pitx2. (B) Down-regulation of eleven selected transcripts was independently confirmed by quantitative RT-PCR at 24, 48 and 72-hpf. dkk2 , a previously reported downstream target of Pitx2 during mouse eye development , was included for comparison. Statistical significance is indicated by asterisks (compared with wild-type values) as follows: a single asterisk corresponds to P < 0.05; two asterisks to P < 0.01; three asterisks to P < 0.001.

Article Snippet: RNA from three independent pitx2 M64 * homozygous eye collections with RNA Integrity Numbers (RINs) of 8.9, 8.6 and 9.0 and three independent wild-type collections with RINs 8.7, 8.9, and 8.7 was submitted to OakLabs (Hennigsdorf, Germany) for transcriptome analysis using an ArrayXS Zebrafish v1 microarray containing 60 023 target IDs.

Techniques: Microarray, Quantitative RT-PCR, Comparison